The Response of Anti-Peptide Serum Antibodies ATLGATLNRLDFNVNNK Derived from S. Flexnneri Protein Sub-Unit Pili toward of Activity the Hemagglutination and Protection from Leaking Solution Enterocyte in Mice

The purpose of this study is whether the antibodies produced from injections of ATLGATLNRLDFNVNNLK (A-K) amino acid found in Bacillus thuringiensis are protective against Shigella infection.

The methods first step is the intraperitoneal injection of A-K amino acid. Furthermore, the antibodies produced sequentially were carried out with an anti hemagglutination test on the hemagglutinin pili subunit 49.8 kDa S. flexneri, dot blot examination, western blot examination and diarrhea effectiveness test in mice using the MILL method.

The results obtained that the anti-body A-Kamino acid inhibited the pili subunit 49.8 kDa S. flexneri. By using the dot blot method found that the effective concentration of 10-5 as the basis for further examination, western blotting test and the effectiveness of diarrhea in mice.

The conclusion of this study is that the results of immunization protection with the A-K amino acids still require continuation if given orally.

Keywords: Shigella flexneri, protein hemaglutinin 49,8 kDa, (A-K) amino acid.